Publications

Mass Spectrometry Services

Kleinow T, Nischang M, Beck A, Kratzer U, Tanwir F, Preiss W, Kepp G, Jeske H.
Three C-terminal phosphorylation sites in the Abutilon mosaic virus movement protein affect symptom development and viral DNA accumulation.
Virology. 2009;390(1):89-101.

Kratzer U, Frank R, Kalbacher H, Biehl B, Wöstemeyer J, Voigt J.
Subunit structure of the vicilin-like globular storage protein of cocoa seeds and the origin of cocoa- and chocolate-specific aroma precursors.
Food Chemistry. 2009;113(4):903-913.

Thedieck C, Kalbacher H, Kratzer U, Lammers R, Stevanovic S, Klein G.
αB-crystallin is a cytoplasmic interaction partner of the kidney-specific cadherin-16.
Journal of Molecular Biology. 2008;378(1):147-153.

Dihazi H, Mueller C, Asif AR, Flad T, Elmaouhoub A, Mueller GA.
Whole cell profiling and identification of galectin-1 as a potential marker of renal cell carcinoma.
Proteomics - Clinical Applications. 2007;1(2):200-214.

Skopeliti M, Kratzer U, Altenberend F, Panayotou G, Kalbacher H, Stevanovic S, Voelter W, Tsitsilonis OE.
Proteomic exploitation on Prothymosin α-induced mononuclear cell activation.
Proteomics. 2007;11(7):1814-1824.

Zaidi N, Herrmann T, Baechle D, Schleicher S, Gogel J, Driessen C, Voelter W, Kalbacher H.
A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles.
The FEBS Journal. 2007;274(12):3138-3149.

Baechle D, Flad T, Cansier A, Steffen H, Schittek B, Tolson J, Herrmann T, Dihazi H, Beck A, Mueller GA, Mueller M, Stevanovic S, Garbe C, Mueller CA, Kalbacher H.
Cathepsin D is present in human eccrine sweat and involved in the postsecretory processing of the antimicrobial peptide DCD-1L.
The Journal of Biological Chemistry. 2006;281(9):5406-5415.

Flad T, Kratzer U, Schmalfuss J.
Massenspektrometrie-basierte Qualitätskontrolle von Protein- und Peptid-Pharmaka.
Laborwelt. 2006;7(1):34-35.

Flad T, Mueller L, Dihazi H, Grigorova V, Bogumil R, Beck A, Thedieck C, Mueller GA, Kalbacher H, Mueller CA.
T cell epitope definition by differential mass spectrometry: identification of a novel, immunogenic HLA-B8 ligand directly from renal cancer tissue.
Proteomics. 2006;6:364-374.

Ketterlinus R, Kratzer U, Hennenlotter J.
Erste ertragreiche „Minen“ im Biomarker-Screening.
Laborwelt. 2006;7(3):29-31.

Knights AJ, Weinzierl AO, Flad T, Guinn BA, Mueller L, Mufti GJ, Stevanovic S, Pawelec G.
A novel MHC-associated Proteinase 3 peptide isolated from primary chronic myeloid leukaemia cells further supports the significance of this antigen for the immunotherapy of myeloid leukaemias.
Leukemia. 2006;20:1067-1072.

Tolson JP, Flad T, Gnau V, Dihazi H, Hennenlotter J, Beck A, Mueller GA, Kuczyl M, Mueller CA.
Differential detection of S100A8 in transitional cell carcinoma of the bladder by pair wise tissue proteomic and immunohistochemical analysis.
Proteomics. 2006;6:697-708

Flad T, Tolson J.
Mass spectrometry meets medical sciences: making headway in molecular diagnostics.
Analytical and Bioanalytical Chemistry. 2005;381(1):24-27.

Tolson J, Bogumil R, Brunst E, Beck H, Elsner R, Humeny A, Kratzin H, Deeg M, Kuczyk M, Mueller GA, Mueller CA, Flad T.
Serum protein profiling by SELDI mass spectrometry: detection of multiple variants of serum amyloid alpha in renal cancer patients.
Laboratory Investigation. 2004;84:845-856.

van Swieten PF, Maehr R, van den Nieuwendijk AM, Kessler BM, Reich M, Wong CS, Kalbacher H, Leeuwenburgh MA, Driessen C, van der Marel GA, Ploegh HL, Overkleeft HS.
Development of an isotope-coded activity-based probe for the quantitative profiling of cysteine proteases.
Bioorganic & Medicinal Chemistry Letters. 2004;14(12):3131-3134.

Flad T, Schiestel T, Brunner H, Tolson J, Ouyang Q, Pawelec G, Mueller GA, Mueller CA, Tovar GE, Beck H.
Development of an MHC-class I peptide selection assay combining nanoparticle technology and matrix-assisted laser desorption/ionisation mass spectrometry.
Journal of Immunological Methods. 2003;283:205-213.

Flad T, Bogumil R, Tolson J, Schittek B, Garbe C, Deeg M, Mueller CA, Kalbacher H.
Detection of dermcidin-derived peptides in sweat by ProteinChip technology.
Journal of Immunological Methods. 2002;270:53-62.

Flad T, Spengler B, Kalbacher H, Brossart P, Baier D, Kaufmann R, Bold P, Metzger S, Bluggel M, Meyer HE, Kurz B, Muller CA.
Direct identification of major histocompatibility complex class I-bound tumor-associated peptide antigens of a renal carcinoma cell line by a novel mass spectrometric method.
Cancer Research. 1998;58:5803-5811.

Halder T, Pawelec G, Kirkin AF, Zeuthen J, Meyer HE, Kun L, Kalbacher H.
Isolation of novel HLA-DR restricted potential tumor-associated antigens from the melanoma cell line FM3.
Cancer Research. 1997;57(15):3238-3244.

Founders and scientists of PANATecs GmbH and/or Zentrum für Klinische Massenspektrometrie GmbH

Analytical Services

Sturzu A, Klose U, Echner H, Beck A, Gharabaghi A, Kalbacher H, Heckl S.
Cellular uptake of cationic gadolinium-DOTA peptide conjugates with and without N-terminal myristoylation.
Amino Acids. 2008 (in press).

Waraich RS, Zaidi N, Moeschel K, Beck A, Weigert C, Voelter W, Kalbacher H, Lehmann R.
Development and precise characterization of phospho-site-specific antibody of Ser(357) of IRS-1: Elimination of cross reactivity with adjacent Ser(358).
Biochemical and biophysical research communications. 2008; 376(1):26-31.

Heckl S, Regenbogen M, Sturzu A, Gharabaghi A, Feil G, Beck A, Echner H, Nagele T.
Value of apoptin's 40-amino-acid-C-terminal fragment for the differentiation between tumor and non-tumor cells.
Apoptosis. 2008;13(4):495-508.

Heckl S, Sturzu A, Regenbogen M, Beck A, Feil G, Gharabaghi A, Echner H.
A novel polyarginine containing Smac peptide conjugate that mediates cell death in tumor and healthy cells.
Medicinal Chemistry 2008; 4(4):348-54.

Heckl S, Sturzu A, Regenbogen M, Beck A, Gharabaghi A, Echner H.
The differential influence of non-iodinated and mono- or diiodinated benzoic acids on cellular and nuclear uptake of the nuclear localization sequence of the SV 40 T antigen.
International Journal of Pharmaceutics. 2008;355(1-2):131-40.

Kessler T, Reich M, Jahn G, Tolosa E, Beck A, Kalbacher H, Overkleeft H, Schempp S, Driessen C.
Human cytomegalovirus infection interferes with major histocompatibility complex type II maturation and endocytic proteases in dendritic cells at multiple levels.
The Journal of General Virology. 2008;89(10):2427-2436.

Beck A, Hillen N, Dolashki A, Stevanovic S, Salvato B, Voelter W, Dolashka-Angelova P.
Oligosaccharide structure of a functional unit RvH(1)-b of Rapana venosa hemocyanin using HPLC/electrospray ionization mass spectrometry.
Biochimie. 2007;89(8):938-949.

von Blume J, Knippschild U, Dequiedt F, Giamas G, Beck A, Auer A, Van Lint J, Adler G, Seufferlein T.
Phosphorylation at Ser244 by CK1 determines nuclear localization and substrate targeting of PKD2.
EMBO J. 2007; 26(22):4619-33.

Beck A, Poeschel S, Oren A, Baechle D, Reich M, Roetzschke O, Falk K, Boehm BO, Youssef S, Kalbacher H, Overkleeft H, Tolosa E, Driessen C.
Interferon-gamma regulates cathepsin G activity in microglia-derived lysosomes and controls the proteolytic processing of myelin basic protein in vitro.
Immunology. 2007;121(1):82-93.

Dolderer B, Echner H, Beck A, Hartmann HJ, Weser U, Luchinat C, Del Bianco C.
Coordination of three and four Cu(I) to the alpha- and beta-domain of vertebrate Zn-metallothionein-1, respectively, induces significant structural changes.
The FEBS Journal. 2007;274(9):2349-2362.

Kraus M, Ruckrich T, Reich M, Gogel J, Beck A, Kammer W, Berkers CR, Burg D, Overkleeft H, Ovaa H, Driessen C. Activity patterns of proteasome subunits reflect bortezomib sensitivity of hematologic malignancies and are variable in primary human leukemia cells.
Leukemia. 2007;21(1):84-92.

Merkel P, Beck A, Muhammad K, Ali SA, Schönfeld C, Voelter W, Duszenko M.
Spermine isolated and identified as the major trypanocidal compound from the snake venom of Eristocophis macmahoni causes autophagy in Trypanosoma brucei.
Toxicon. 2007;50(4):457-469.

Ali SA, Wang B, Alam M, Beck A, Stoeva S, Voelter W, Abbasi A, Duszenko M.
Structure-activity relationship of an alpha-toxin Bs-Tx28 from scorpion (Buthus sindicus) venom suggests a new alpha-toxin subfamily.
Archives of Biochemistry and Biophysics. 2006;445(1):81-94.

Baechle D, Flad T, Cansier A, Steffen H, Schittek B, Tolson J, Herrmann T, Dihazi H, Beck A, Mueller GA, Mueller M, Stevanovic S, Garbe C, Mueller CA, Kalbacher H.
Cathepsin D is present in human eccrine sweat and involved in the postsecretory processing of the antimicrobial peptide DCD-1L.
The Journal of Biological Chemistry. 2006;281(9):5406-5415.

Figarella K, Uzcategui NL, Beck A, Schoenfeld C, Kubata BK, Lang F, Duszenko M.
Prostaglandin-induced programmed cell death in Trypanosoma brucei involves oxidative stress.
Cell Death and Differentiation. 2006;13(10):1802-1814.

Flad T, Mueller L, Dihazi H, Grigorova V, Bogumil R, Beck A, Thedieck C, Mueller GA, Kalbacher H, Mueller CA.
T cell epitope definition by differential mass spectrometry: identification of a novel, immunogenic HLA-B8 ligand directly from renal cancer tissue.
Proteomics. 2006;6(1):364-374.

Hennige AM, Stefan N, Kapp K, Lehmann R, Weigert C, Beck A, Moeschel K, Mushack J, Schleicher E, Haring HU.
Leptin down-regulates insulin action through phosphorylation of serine-318 in insulin receptor substrate 1.
The FASEB Journal. 2006;20(8):1206-1208.

Klimentzou P, Paravatou-Petsotas M, Zikos C, Beck A, Skopeliti M, Czarnecki J, Tsitsilonis O, Voelter W, Livaniou E, Evangelatos GP.
Development and immunochemical evaluation of antibodies Y for the poorly immunogenic polypeptide prothymosin alpha.
Peptides. 2006;27(1):183-193.

Ruckrich T, Brandenburg J, Cansier A, Muller M, Stevanovic S, Schilling K, Wiederanders B, Beck A, Melms A, Reich M, Driessen C, Kalbacher H.
Specificity of human cathepsin S determined by processing of peptide substrates and MHC class II-associated invariant chain.
Biological Chemistry. 2006;387(10-11):1503-1511.

Skopeliti M, Voutsas IF, Klimentzou P, Tsiatas ML, Beck A, Bamias A, Moraki M, Livaniou E, Neagu M, Voelter W, Tsitsilonis OE.
The immunologically active site of prothymosin alpha is located at the carboxy-terminus of the polypeptide. Evaluation of its in vitro effects in cancer patients.
Cancer Immunology, Immunotherapy. 2006;55(10):1247-1257.

Tolson JP, Flad T, Gnau V, Dihazi H, Hennenlotter J, Beck A, Mueller GA, Kuczyk M, Mueller CA.
Differential detection of S100A8 in transitional cell carcinoma of the bladder by pair wise tissue proteomic and immunohistochemical analysis.
Proteomics. 2006;6(2):697-708.

Burster T, Beck A, Tolosa E, Schnorrer P, Weissert R, Reich M, Kraus M, Kalbacher H, Haring HU, Weber E, Overkleeft H, Driessen C.
Differential processing of autoantigens in lysosomes from human monocyte-derived and peripheral blood dendritic cells.
Journal of Immunology. 2005 Nov 1;175(9):5940-5949.

Mussig K, Staiger H, Fiedler H, Moeschel K, Beck A, Kellerer M, Haring HU.
Shp2 is required for protein kinase C-dependent phosphorylation of serine 307 in insulin receptor substrate-1.
The Journal of Biological Chemistry. 2005 Sep 23;280(38):32693-32699.

Weigert C, Hennige AM, Brischmann T, Beck A, Moeschel K, Schauble M, Brodbeck K, Haring HU, Schleicher ED, Lehmann R.
The phosphorylation of Ser318 of insulin receptor substrate 1 is not per se inhibitory in skeletal muscle cells but is necessary to trigger the attenuation of the insulin-stimulated signal.
The Journal of Biological Chemistry. 2005;280(45):37393-37399.

Burster T, Beck A, Tolosa E, Marin-Esteban V, Rotzschke O, Falk K, Lautwein A, Reich M, Brandenburg J, Schwarz G, Wiendl H, Melms A, Lehmann R, Stevanovic S, Kalbacher H, Driessen C.
Cathepsin G, and not the asparagine-specific endoprotease, controls the processing of myelin basic protein in lysosomes from human B lymphocytes.
Immunology. 2004;172(9):5495-5503.

Dolashka-Angelova P, Beck A, Dolashki A, Stevanovic S, Beltramini M, Salvato B, Hristova R, Velkova L, Voelter W. Carbohydrate moieties of molluscan Rapana venosa hemocyanin.
Micron. 2004;35(1-2):101-104.

Moeschel K, Beck A, Weigert C, Lammers R, Kalbacher H, Voelter W, Schleicher ED, Haring HU, Lehmann R. Protein kinase C-zeta-induced phosphorylation of Ser318 in insulin receptor substrate-1 (IRS-1) attenuates the interaction with the insulin receptor and the tyrosine phosphorylation of IRS-1.
The Journal of Biological Chemistry. 2004;279(24):25157-25163.

Beck A, Moeschel K, Deeg M, Haring HU, Voelter W, Schleicher ED, Lehmann R.
Identification of an in vitro insulin receptor substrate-1 phosphorylation site by negative-ion muLC/ES-API-CID-MS hybrid scan technique.
Journal of the American Society for Mass Spectrometry. 2003;14(4):401-405.

Dolashka-Angelova P, Beck A, Dolashki A, Beltramini M, Stevanovic S, Salvato B, Voelter W.
Characterization of the carbohydrate moieties of the functional unit RvH1-a of Rapana venosa haemocyanin using HPLC/electrospray ionization MS and glycosidase digestion.
The Biochemical journal. 2003;374(Pt 1):185-192.

Friess U, Beck A, Kohne E, Lehmann R, Koch S, Haring HU, Schmuelling RM, Schleicher E.
Novel hemoglobin variant [beta66(E10) Lys-->Asn], with decreased oxygen affinity, causes falsely low hemoglobin A1c values by HPLC.
Clinical chemistry. 2003;49(8):1412-1415.

Lehmann R, Friess U, Haring HU, Voelter W, Liebich H, Beck A.
Investigation of a capillary electrophoretic approach for direct quantification of apolipoprotein A-I in serum.
Electrophoresis. 2003;24(9):1422-1428.

Beck A, Deeg M, Moeschel K, Schmidt EK, Schleicher ED, Voelter W, Haring HU, Lehmann R.
Alkaline liquid chromatography/electrospray ionization skimmer collision-induced dissociation mass spectrometry for phosphopeptide screening.
Rapid Communications in Mass Spectrometry. 2001;15(23):2324-2333.

Bossenmaier B, Strack V, Stoyanov B, Krutzfeldt J, Beck A, Lehmann R, Kellerer M, Klein H, Ullrich A, Lammers R, Haring HU.
Serine residues 1177/78/82 of the insulin receptor are required for substrate phosphorylation but not autophosphorylation.
Diabetes. 2000;49(6):889-895.

Lehmann R, Huber M, Beck A, Schindera T, Rinkler T, Houdali B, Weigert C, Haring HU, Voelter W, Schleicher ED.
Simultaneous, quantitative analysis of UDP-N-acetylglucosamine, UDP-N-acetylgalactosamine, UDP-glucose and UDP-galactose in human peripheral blood cells, muscle biopsies and cultured mesangial cells by capillary zone electrophoresis.
Electrophoresis. 2000;21(14):3010-3015.

Voelter W, Stoeva S, Echner H, Beck A, Schütz J, Lehmann R, Häring HU, Schleicher E, Mullen AM, Casserly U, Troy DJ, Tsitsilonis OE, Lymberi P, Baxevanis CN, Papamichail M.
Analytical tools for rapid, sensitive, quantitative identification of potential meat quality markers.
Journal für praktische Chemie. 2000;342:179-191.

Beck A, Lehmann R, Gambaro G, Haring HU, Schleicher ED, Voelter W, Ceol M.
Advances in reverse transcription polymerase chain reaction analysis of cellular mRNA levels of transforming growth factor-beta1 by capillary electrophoresis with laser-induced fluorescence detection.
Clinical Chemistry and Laboratory Medicine. 1999;37(5):527-532.

Founders and scientists of PANATecs GmbH and/or Zentrum für Klinische Massenspektrometrie GmbH

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