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Liquid Chromatography

HILIC Chromatography

HILIC chromatography serves to separate hydrophilic analytes, such as glycans, after their release from glycoproteins.  By using fluorescence labeling, quantitative batch-specific glycan profiles can be created. These are important in the context of comparability analyses. Like RP-HPLC, this chromatography can be coupled online to ESI mass spectrometry, for the direct identification of the analytes.

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